(G.E. Sosinsky, University of California at San Diego; California; USA Cat# Mo503, RRID:AB_10013323)Copy Citation Copied
URL: http://antibodyregistry.org/AB_10013323
Proper Citation: (G.E. Sosinsky, University of California at San Diego; California; USA Cat# Mo503, RRID:AB_10013323)
Target Antigen: Panx1
Host Organism: mouse
Clonality: monoclonal
Comments: This monoclonal antibody was generated by Abgent Inc San Diego, CA for the Sosinsky laboratory against a peptide containing amino acids 17 to 31 LKEPTEPKFKGLRLE of the N terminus of Panx1 and denoted as Mo503 This sequence is invariant among mouse rat and human sequences
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No rating or validation information has been found for Abgent Mouse 503 anti Pannexin1.
Possibly Non-Specific
Extracted Antibody Information: "and (4) mouse anti-Panx1 (denoted as Mo503; NIF antibody registry number: AB_10013323)."
Extracted Specificity Statement: "Tests of these antibodies on tissue lysates from two different Panx1 KO mice demonstrate that they recognize Panx1 bands, however additional bands are labeled in the KO for some of the antibodies and these vary between KO animals. Thus, it is still unclear whether differences seen between the four antibodies are due to different epitopes being recognized, non-specificity, or the presence of some residual Panx1 protein in KO tissues. Nevertheless, scientific papers continue to be published with these antibodies and/or these KO mouse models, so we discuss strategies for publishing this kind of data by recommending several standards."
Data was mined by Antibody Watch (https://arxiv.org/pdf/2008.01937.pdf), from PMID:23390418
Possibly Non-Specific
Extracted Antibody Information: "and (4) mouse anti-Panx1 (denoted as Mo503; NIF antibody registry number: AB_10013323)."
Extracted Specificity Statement: "We showed using Panx1 KO mice as a negative control (Figures 3B,C) that these four antibodies recognize Panx1 at its expected sizes as well as some sizes that are not yet explainable. There are some non-specific bands on some of the Western blots that do not disappear in the knock out tissue lysates consistently, as they are only present in some tissue samples. It is worth noting that Qiu et al."
Data was mined by Antibody Watch (https://arxiv.org/pdf/2008.01937.pdf), from PMID:23390418
Source: Antibody Registry
