(Amparo Estepa Lab University Miguel Hernandez; Spain Cat# Anti-Mx3, RRID:AB_2716267)Copy Citation Copied
URL: http://antibodyregistry.org/AB_2716267
Proper Citation: (Amparo Estepa Lab University Miguel Hernandez; Spain Cat# Anti-Mx3, RRID:AB_2716267)
Target Antigen: Mx3
Host Organism: rabbit
Clonality: polyclonal
Comments: Chico et al, 2010 "Production of anti-trout Mx sera in rabbit. The 15-mer peptide corresponding to amino acid residues 209 to 223 of the rainbow trout Mx3 protein (GenBank accession number U47946) was synthesized by Sigma to a purity of >95%, as determined by high-performance liquid chromatography and mass spectrophotometry. To obtain the antisera, rabbits were first injected with 1 mg/ml of synthetic peptide diluted 1:1 in Freund's complete adjuvant. Four weeks later, a second injection with the same antigen in Freund's incomplete adjuvant was given. Blood samples were collected before injection (preimmune sera) and 20 days after the second injection. The blood samples were then incubated for 2 h at 4°C and centrifuged to obtain the sera."
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