URL: http://www.genome.duke.edu/labs/ohler/research/PARalyzer/
Proper Citation: PARalyzer (RRID:SCR_001208)
Description: Software tool to generate a high resolution map of interaction sites between RNA-binding proteins and their targets. The algorithm utilizes the deep sequencing reads generated by the newly developed PAR-CLIP (Photoactivatable-Ribonucleoside-Enhanced Crosslinking and Immunoprecipitation) protocol. The use of photoactivatable nucleotides in the PAR-CLIP protocol results in a more efficient crosslinking between the RNA-binding protein and its target relative to other CLIP methods; in addition a nucleotide substitution occurs at the site of crosslinking during Illumina library preparation. PARalyzer utilizes this nucleotide substition in a kernel density estimate classifier to generate the high resolution set of Protein-RNA interaction sites.
Abbreviations: PARalyzer
Synonyms: PAR-CLIP data analyzer, PARalyzer (PAR-CLIP data analyzer)
Resource Type: software resource
Defining Citation: PMID:21851591
Keywords: interaction, rna-binding protein, bio.tools
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