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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 10846 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepGEX-4T1
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Backbone manufacturerAmersham
- Backbone size w/o insert (bp) 4969
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHPV-16 E2
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Alt nameE2
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SpeciesHuman papillomavirus
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GenBank IDNP_041328
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Entrez GeneE2 (a.k.a. HpV16gp4)
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Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI* (not destroyed)
- 3′ cloning site Possibly EcoRI (not destroyed)
- 5′ sequencing primer pGEX5' (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
HPV-16 E2 (aa1-365) from pJG4-5-derived plasmid. See author's map.
*Note that Addgene's sequence and sequence we have received from requesting labs suggest that the cloning strategy described in the depositor map is not accurate. There is a BamHI site at the 5' of the insert and an EcoRI site at the 3' end.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p3187 HPV-16 E2 was a gift from Peter Howley (Addgene plasmid # 10846 ; http://n2t.net/addgene:10846 ; RRID:Addgene_10846) -
For your References section:
Mapping and characterization of the interaction domains of human papillomavirus type 16 E1 and E2 proteins. Yasugi T, Benson JD, Sakai H, Vidal M, Howley PM. J Virol. 1997 Feb . 71(2):891-9. PubMed 8995605
Map uploaded by the depositor.
