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PurposeHis-tagged TEV protease with solubility increasing mutations
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 125194 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepTH24 based on pDEST42
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Backbone manufacturerInvitrogen
- Total vector size (bp) 6536
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameTEV_SH
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SpeciesTobacco etch virus
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Insert Size (bp)888
- Promoter T7lac
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Tag
/ Fusion Protein
- V5 His-tag (C terminal on backbone)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7term (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Addgene Notes
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that this plasmid may run a dimer (>13kb) or multimer.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
TEVSH was a gift from Helena Berglund (Addgene plasmid # 125194 ; http://n2t.net/addgene:125194 ; RRID:Addgene_125194) -
For your References section:
Improved solubility of TEV protease by directed evolution. van den Berg S, Lofdahl PA, Hard T, Berglund H. J Biotechnol. 2006 Feb 10;121(3):291-8. doi: 10.1016/j.jbiotec.2005.08.006. Epub 2005 Sep 15. 10.1016/j.jbiotec.2005.08.006 PubMed 16150509
