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PurposeMammalian expression vector for expression of the SARS-CoV-2 spike HexaPro variant
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 154754 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonepαH
- Backbone size w/o insert (bp) 4746
- Total vector size (bp) 8370
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSpike (HexaPro variant)
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SpeciesSevere acute respiratory syndrome coronavirus 2
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Insert Size (bp)3624
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MutationEctodomain only (AAs 1-1208); 682-685 (furin site) replaced with 'GSAS'; F817P, A892P, A899P, A942P, K986P, V987P
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GenBank IDMN908947
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Entrez GeneS (a.k.a. GU280_gp02, spike glycoprotein)
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Tags
/ Fusion Proteins
- HRV 3C cleavage site (before tags) (C terminal on backbone)
- 8X His tag (C terminal on backbone)
- 2X Strep-Tag II (C terminal on backbone)
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SARS-CoV-2 S HexaPro was a gift from Jason McLellan (Addgene plasmid # 154754 ; http://n2t.net/addgene:154754 ; RRID:Addgene_154754) -
For your References section:
Structure-based design of prefusion-stabilized SARS-CoV-2 spikes. Hsieh CL, Goldsmith JA, Schaub JM, DiVenere AM, Kuo HC, Javanmardi K, Le KC, Wrapp D, Lee AG, Liu Y, Chou CW, Byrne PO, Hjorth CK, Johnson NV, Ludes-Meyers J, Nguyen AW, Park J, Wang N, Amengor D, Lavinder JJ, Ippolito GC, Maynard JA, Finkelstein IJ, McLellan JS. Science. 2020 Sep 18;369(6510):1501-1505. doi: 10.1126/science.abd0826. Epub 2020 Jul 23. 10.1126/science.abd0826 PubMed 32703906
Map uploaded by the depositor.
