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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 16459 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCMV-Neo-XhoI
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Backbone manufacturerVogelstein Lab
- Backbone size w/o insert (bp) 6600
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDCC
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SpeciesH. sapiens (human)
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Insert Size (bp)4400
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Entrez GeneDCC (a.k.a. CRC18, CRCR1, HGPPS2, IGDCC1, MRMV1, NTN1R1)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Addgene Notes
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Additional reference: Cho KR et al, The DCC Gene: Structural analysis and mutations in colorectal carcinomas. Genomics 19: 525-531, 1994.
Addgene NGS results found DCC isoform X1 [XP_016881057.1] with an F23L variant.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV DCC was a gift from Bert Vogelstein (Addgene plasmid # 16459 ; http://n2t.net/addgene:16459 ; RRID:Addgene_16459) -
For your References section:
Identification of a chromosome 18q gene that is altered in colorectal cancers. Fearon ER, Cho KR, Nigro JM, Kern SE, Simons JW, Ruppert JM, Hamilton SR, Preisinger AC, Thomas G, Kinzler KW. Science. 1990 Jan 5. 247(4938):49-56. 10.1126/science.2294591 PubMed 2294591
Map uploaded by the depositor.
