pEZYmyc
(Plasmid
#18702)
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Purpose(Empty Backbone) pCMV/myc/cyto converted Gateway Destination vector for expressing C-terminal myc tagged fustion proteins in mammalian cells
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 18702 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMV/myc/cyto
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Backbone manufacturerInvitrogen
- Backbone size (bp) 7150
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Modifications to backboneGateway cassette was inserted into original vector to create the pEZYmyc Gateway Destination vector
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)ccdB Survival
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNone
- Promoter CMV
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Tag
/ Fusion Protein
- Myc (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
pCMV/myc/cyto Gateway-compatible Destination vector. Combine with an Entry clone containing your gene of interest with the Gateway LR reaction to generate an expression clone containing a C-terminal myc tag.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEZYmyc was a gift from Yu-Zhu Zhang (Addgene plasmid # 18702) -
For your References section:
An in vitro recombination method to convert restriction- and ligation-independent expression vectors. Guo F, Chiang MY, Wang Y, Zhang YZ. Biotechnol J. 2008 Mar . 3(3):370-7. 10.1002/biot.200700170 PubMed 18064608