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Purpose(Empty Backbone)
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 27553 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCMV4
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Backbone manufacturerDr. David Russell, UTSW
- Backbone size (bp) 4874
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Vector typeMammalian Expression
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Tag
/ Fusion Protein
- 3x HA
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NA (unknown if destroyed)
- 3′ cloning site NA (unknown if destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer hGH-pA-R (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid carries three copies of the influenza hemagglutinin (HA) epitope tag (YPYDVPDYA) together with restriction sites for in-frame cloning of cDNA inserts.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV4-HA was a gift from Shao-Cong Sun (Addgene plasmid # 27553 ; http://n2t.net/addgene:27553 ; RRID:Addgene_27553) -
For your References section:
Negative regulation of the nuclear factor kappa B-inducing kinase by a cis-acting domain. Xiao G, Sun SC. J Biol Chem. 2000 Jul 14. 275(28):21081-5. 10.1074/jbc.M002552200 PubMed 10887201
