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pCSCMV:tdTomato
(Plasmid #30530)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 30530 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Cloning Grade DNA 30530-DNA.cg 2 µg of cloning grade DNA in Tris buffer 1 $105

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCSGFP2
  • Backbone size w/o insert (bp) 4100
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    tdTomato
  • Species
    synthetic
  • Insert Size (bp)
    1431
  • Mutation
    R415H
  • GenBank ID
    AY678269

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that there are some discrepancies between Addgene's quality control sequence and the assembled sequence from the depositor, including a H415R mutation in the downstream dimer component of tdTomato. This mutation may impact tdTomato expression.

Information for Cloning Grade DNA (Catalog # 30530-DNA.cg) ( Back to top )

Purpose

Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

Delivery

  • Amount 2 µg
  • Guaranteed Concentration 100 ng/µl +/- 5 ng/µl
  • Pricing $105 USD
  • Storage DNA can be stored at 4℃ (short term) or -20℃ (long term).

Terms and Licenses

Quality Control

Addgene has verified this plasmid using Next Generation Sequencing. Results are available here

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCSCMV:tdTomato was a gift from Gerhart Ryffel (Addgene plasmid # 30530 ; http://n2t.net/addgene:30530 ; RRID:Addgene_30530)
  • For your References section:

    Red fluorescent Xenopus laevis: a new tool for grafting analysis. Waldner C, Roose M, Ryffel GU. BMC Dev Biol. 2009 . 9():37. 10.1186/1471-213X-9-37 PubMed 19549299