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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 31057 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backboneModified pEGFP-N1 (see note)
- Backbone size w/o insert (bp) 4000
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAnkyrin B
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Alt nameankyrin 2
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SpeciesH. sapiens (human)
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Entrez GeneANK2 (a.k.a. ANK-2, CFAP87, FAP87, LQT4, brank-2)
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Tag
/ Fusion Protein
- 2XHA (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV-Fwd (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
HA-ankyrin-B was prepared based on an ankyrin-B-GFP template in a pEGFP-N1 vector (Mohler et al., 2002). GFP was cut out between the PmeI and the NotI sites and replaced with a 2XHA-tag encoding sequence (YPYDVPDYA) with a PmeI site on its 5′ end and a stop codon followed by a NotI site on the 3′ end that was synthesized by PCR.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AnkyrinB-2XHA was a gift from Vann Bennett (Addgene plasmid # 31057 ; http://n2t.net/addgene:31057 ; RRID:Addgene_31057) -
For your References section:
Ankyrin-B interactions with spectrin and dynactin-4 are required for dystrophin-based protection of skeletal muscle from exercise injury. Ayalon G, Hostettler JD, Hoffman J, Kizhatil K, Davis JQ, Bennett V. J Biol Chem. 2011 Mar 4. 286(9):7370-8. 10.1074/jbc.M110.187831 PubMed 21186323
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