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pLew100_myc_BirA*
(Plasmid #41716)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 41716 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLew100
  • Backbone size (bp) 7417
  • Modifications to backbone
    Contains myc-BirA* module (R118G)
  • Vector type
    T. brucei expression
  • Promoter PARP
  • Selectable markers
    Phleomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The pLew100_myc-BirA* plasmid is derived from the pcDNA3.1(-)_myc-BirA* (http://www.addgene.org/36047/) plasmid originating in the Roux lab (Sanford Health, SD, USA). It contains the myc-BirA* module cloned in the T. brucei pLew100 expression vector, with some extra restriction sites to facilitate cloning.
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

If you use this plasmid in a publication, please also cite the Morriswood paper as well as the following article:
A promiscuous biotin ligase fusion protein identifies proximal and interacting proteins in mammalian cells. Roux et al (J Cell Biol. 2012 Mar 12). PubMed - https://www.ncbi.nlm.nih.gov/pubmed/22412018 .

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLew100_myc_BirA* was a gift from Graham Warren (Addgene plasmid # 41716 ; http://n2t.net/addgene:41716 ; RRID:Addgene_41716)
  • For your References section:

    Novel bilobe components in Trypanosoma brucei identified using proximity-dependent biotinylation. Morriswood B, Havlicek K, Demmel L, Yavuz S, Sealey-Cardona M, Vidilaseris K, Anrather D, Kostan J, Djinovic-Carugo K, Roux K, Warren G. Eukaryot Cell. 2012 Dec 21. 10.1128/EC.00326-12 PubMed 23264645