5HRE/GFP
(Plasmid #46926)

Ordering

This material is available to academics and nonprofits only.

Backbone

• Vector backbone
  pEF/myc/cyto
• Backbone manufacturer
  Invitrogen
• Backbone size w/o insert (bp) 5500
• Vector type
  Mammalian Expression
• Selectable markers
  Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s)
  Ampicillin, 100 μg/mL
• Growth Temperature
  37°C
• Growth Strain(s)
  DH5alpha
• Copy number
  Unknown

Gene/Insert

• Gene/Insert name
  5X HRE of VEGF
• Alt name
  VEGFA
• Alt name
  VEGF
• Species
  H. sapiens (human)
• Mutation
  five copies of a 35-bp fragment from the hypoxia-responsive element (HRE) of the human VEGF gene and a human cytomegalovirus minimal promoter
• Entrez Gene
  VEGFA (a.k.a. L-VEGF, MVCD1, VEGF, VPF)
• Promoter EF-1α promoter
• Tag / Fusion Protein
  • d2EGFP (C terminal on insert)

Cloning Information

• Cloning method Restriction Enzyme
• 5′ cloning site KpnI (not destroyed)
• 3′ cloning site NotI (not destroyed)
• 5′ sequencing primer EF1a-F
• 3′ sequencing primer BGHrev
(Common Sequencing Primers)

Resource Information

• Articles Citing this Plasmid
  • 19 References

Terms and Licenses

• Academic/Nonprofit Terms
  • UBMTA
• Industry Terms
  • Not Available to Industry

Depositor Comments