-
PurposeInhibits dynein-dynactin interaction in mammalian cells
-
Depositing Lab
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 51146 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbonepDsRed-N1
-
Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4700
- Total vector size (bp) 5700
-
Vector typeMammalian Expression
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)XL10 Gold
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameDCTN1
-
Alt namep150Glued
-
SpeciesG. gallus (chicken)
-
Insert Size (bp)997
-
MutationQ118R, E340K
-
GenBank IDXM_015301318.1
-
Entrez GeneDCTN1 (a.k.a. Chip150)
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer unknown
- 3′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
-
Articles Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that mutations Q118R and E340K were found during Addgene's quality control. The depositor noted that these mutations do NOT affect plasmid function.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
DsRed-p150 217–548 was a gift from Trina Schroer (Addgene plasmid # 51146 ; http://n2t.net/addgene:51146 ; RRID:Addgene_51146) -
For your References section:
Distinct cell cycle-dependent roles for dynactin and dynein at centrosomes. Quintyne NJ, Schroer TA. J Cell Biol. 2002 Oct 28;159(2):245-54. Epub 2002 Oct 21. 10.1083/jcb.200203089 PubMed 12391026