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pCAG-HA-rat Nrxn1alpha
(Plasmid #58266)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 58266 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCAGGS
  • Backbone size w/o insert (bp) 4869
  • Total vector size (bp) 9303
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Nrxn1alpha
  • Alt name
    neurexin 1alpha
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    4434
  • Mutation
    see comments
  • GenBank ID
    NM_021767
  • Entrez Gene
    Nrxn1 (a.k.a. Nrxn1b)
  • Promoter CAG
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer pCAG-F (5'-GCAACGTGCTGGTTATTGTG-3')
  • 3′ sequencing primer Bglob-pA-R (5'-TTTTGGCAGAGGGAAAAAGA-3'); pCAG_rev_seq (5'-CAGCCACCACCTTCTGATAG-3')
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See map & sequence file for insert-releasing enzymes (e.g. NheI / XmaI).
Insert can also be released without HA-tag (e.g. by SpeI / XmaI digest).
The rat neurexin 1 alpha encoded by this plasmid lacks alternatively spliced segment 4 (AS4), and contains the regions homologous to alternatively spliced segment 3 and 5 (AS3 and AS5, respectively) of mouse neurexin 1 alpha (compare Schreiner et al., Neuron, 2014 [DOI: 10.1016/j.neuron.2014.09.011]).

Note: HA-epitope tag was introduced at the mature N-terminus.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-HA-rat Nrxn1alpha was a gift from Peter Scheiffele (Addgene plasmid # 58266 ; http://n2t.net/addgene:58266 ; RRID:Addgene_58266)
  • For your References section:

    Silencing of neuroligin function by postsynaptic neurexins. Taniguchi H, Gollan L, Scholl FG, Mahadomrongkul V, Dobler E, Limthong N, Peck M, Aoki C, Scheiffele P. J Neurosci. 2007 Mar 14;27(11):2815-24. 10.1523/JNEUROSCI.0032-07.2007 PubMed 17360903