pBrain-GFP-shGL2
(Plasmid
#60004)
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PurposeCo-expresses control GL2 shRNA and GFP.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 60004 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepBrain
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Backbone manufacturerStephen J Royle
- Backbone size w/o insert (bp) 4733
- Total vector size (bp) 5447
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Modifications to backboneInclusion of control shRNA to target GL2 (firefly luciferase).
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Vector typeMammalian Expression, RNAi
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)XL10 Gold
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameEGFP
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gRNA/shRNA sequenceshGL2 - CGTACGCGGAATACTTCGA
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SpeciesA. victoria (Jellyfish), Photinus pyralis (Firefly)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site ApaLI (not destroyed)
- 3′ cloning site NheI (not destroyed)
- 5′ sequencing primer -
- 3′ sequencing primer - (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBrain-GFP-shGL2 was a gift from Stephen Royle (Addgene plasmid # 60004 ; http://n2t.net/addgene:60004 ; RRID:Addgene_60004) -
For your References section:
A TACC3/ch-TOG/clathrin complex stabilises kinetochore fibres by inter-microtubule bridging. Booth DG, Hood FE, Prior IA, Royle SJ. EMBO J. 2011 Mar 2;30(5):906-19. doi: 10.1038/emboj.2011.15. Epub 2011 Feb 4. 10.1038/emboj.2011.15 PubMed 21297582
Map uploaded by the depositor.
