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PurposeExpression of Ricin A Chain (RTA) in E. coli
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 64029 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Currently unavailable outside the U.S.
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepET23a
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Backbone manufacturerNovagen
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameRicin A Chain
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Alt nameRTA
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Alt nameRicin Toxin A chain
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SpeciesRicinus communis
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Tag
/ Fusion Protein
- none
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Ricin Toxin A Chain (RTA) is the catalytic domain of the ricin toxin. Due to its lack of the B-chain necessary for cell entry, RTA alone in solution is generally not considered a toxic protein to animals and humans.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
RTA was a gift from Vern Schramm (Addgene plasmid # 64029 ; http://n2t.net/addgene:64029 ; RRID:Addgene_64029) -
For your References section:
Transition state analogues in structures of ricin and saporin ribosome-inactivating proteins. Ho MC, Sturm MB, Almo SC, Schramm VL. Proc Natl Acad Sci U S A. 2009 Dec 1;106(48):20276-81. doi: 10.1073/pnas.0911606106. Epub 2009 Nov 17. 10.1073/pnas.0911606106 PubMed 19920175