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Purpose(Empty Backbone) Third generation lentiviral vector expressing GFP and puromycin
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 73582 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
| Cloning Grade DNA | 73582-DNA.cg | 2 µg of cloning grade DNA in Tris buffer | 1 | $105 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepLenti-CMV-MCs-SV-puro
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Backbone manufacturergift from Dr. Hong Zhang, University of Massachusetts Medical School
- Backbone size (bp) 8829
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Modifications to backboneGFP insert
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Vector typeLentiviral
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Selectable markersPuromycin
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Tag
/ Fusion Protein
- GFP tag (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer GAGCACCCAGTCCGCCCTG (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Information for Cloning Grade DNA (Catalog # 73582-DNA.cg) ( Back to top )
Purpose
Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.
Delivery
- Amount 2 µg
- Guaranteed Concentration 100 ng/µl +/- 5 ng/µl
- Pricing $105 USD
- Storage DNA can be stored at 4℃ (short term) or -20℃ (long term).
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Addgene has verified this plasmid using Next Generation Sequencing. Results are available here
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLenti-CMV-MCS-GFP-SV-puro was a gift from Paul Odgren (Addgene plasmid # 73582 ; http://n2t.net/addgene:73582 ; RRID:Addgene_73582) -
For your References section:
Studies of OC-STAMP in Osteoclast Fusion: A New Knockout Mouse Model, Rescue of Cell Fusion, and Transmembrane Topology. Witwicka H, Hwang SY, Reyes-Gutierrez P, Jia H, Odgren PE, Donahue LR, Birnbaum MJ, Odgren PR. PLoS One. 2015 Jun 4;10(6):e0128275. doi: 10.1371/journal.pone.0128275. eCollection 2015. PONE-D-14-50827 [pii] PubMed 26042409
