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Purposemammalian expression of GADD34
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 75478 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepXJ40
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGADD34
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Alt nameprotein phosphatase 1 regulatory subunit 15A
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SpeciesH. sapiens (human)
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Insert Size (bp)2022
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Entrez GenePPP1R15A (a.k.a. GADD34)
- Promoter CMV
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Tag
/ Fusion Protein
- FLAG (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site KpnI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byMatt Brush
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Our sequencing results with T7 (5'-TAATACGACTCACTATAGGG-3') found extra SGSKL amino acids immediately following the FLAG tag and before the start of the GADD34 coding fragment. The depositor states that this discrepancy does not affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pXJ40 GADD34 Wt (1-674aa) was a gift from Shirish Shenolikar (Addgene plasmid # 75478 ; http://n2t.net/addgene:75478 ; RRID:Addgene_75478) -
For your References section:
Structural and Functional Analysis of the GADD34:PP1 eIF2alpha Phosphatase. Choy MS, Yusoff P, Lee IC, Newton JC, Goh CW, Page R, Shenolikar S, Peti W. Cell Rep. 2015 Jun 30;11(12):1885-91. doi: 10.1016/j.celrep.2015.05.043. Epub 2015 Jun 18. 10.1016/j.celrep.2015.05.043 PubMed 26095357