LentiCRISPRv2-mCherry
(Plasmid #99154)

Ordering

This material is available to academics and nonprofits only.

Backbone

• Vector backbone
  LentiCRISPRv2
• Backbone manufacturer
  Feng Zhang
• Backbone size w/o insert (bp) 10000
• Total vector size (bp) 14984
• Modifications to backbone
  mCherry P2A-linked to hSpCAS9 by InFusion cloning.
• Vector type
  Mammalian Expression, Lentiviral, CRISPR
• Selectable markers
  mCherry

Growth in Bacteria

• Bacterial Resistance(s)
  Ampicillin, 100 μg/mL
• Growth Temperature
  37°C
• Growth Strain(s)
  NEB Stable
• Growth instructions
  Use SapI digest to check for unwanted recombination of lentiviral plasmid. Only amplify in RecA- bacteria (eg. Stbl3).
• Copy number
  High Copy

Gene/Insert 1

• Gene/Insert name
  Cas9
• Alt name
  S. pyogenes CRISPR-Cas9
• Species
  Synthetic
• Insert Size (bp)
  4200
• Promoter EFS-NS
• Tag / Fusion Protein
  • FLAG (C terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method Restriction Enzyme
• 5′ cloning site AgeI, XbaI, AfeI (not destroyed)
• 3′ cloning site BamHI (not destroyed)
• 5′ sequencing primer hEF1aprom-F
• 3′ sequencing primer WPRE-R
(Common Sequencing Primers)

Gene/Insert 2

• Gene/Insert name
  mCherry
• Species
  Discoma sp.
• Insert Size (bp)
  708
• Promoter EFS-NS

Cloning Information for Gene/Insert 2

• Cloning method Ligation Independent Cloning
• 5′ sequencing primer ACC AAA GAG GTG CTG GAC G
• 3′ sequencing primer WPRE-R
(Common Sequencing Primers)

Resource Information

• Supplemental Documents
  • LentiCRISPRv2_mCherry.gb
  • Cutting validation data post-FACS sort
• A portion of this plasmid was derived from a plasmid made by
  LentiCRISPRv2 was produced by Feng Zhang, MIT. mCherry is the copyright of Clontech.
• Articles Citing this Plasmid
  • 24 References

Terms and Licenses

• Academic/Nonprofit Terms
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms
  • Not Available to Industry

Depositor Comments