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Nucleotide sequence of the major DNA-binding protein gene of herpes simplex virus type 2 and a comparison with the type 1 counterpart

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Summary

The nucleotide sequence of a region encompassing about 5,200 base pairs (bp) of the left side of the origin of replication in the long unique region of the herpes simplex virus type 2 (HSV-2) has been determined. This region contained the major DNA-binding protein or the infected-cell protein 8 (ICP 8) gene and 5′-part of the counterpart of HSV-1 ICP 18.5 gene. A comparison of the nucleotide sequence of the ICP 8 gene between HSV-1 and HSV-2 showed an 89.8% homology. A primer extension analysis for the HSV-2 ICP 8 mRNA showed that the major transcriptional start site was mapped at 315 bp upstream of the initiation codon. A comparison of the predicted functional amino acid sequence of the ICP 8 between HSV-1 and HSV-2 revealed a striking homology (97.2%), the value of which was the highest among those of the other poly-peptides encoded by HSV-1 and HSV-2. Some domains, which were shown to be required for the nuclear function, the binding to single-stranded DNA and the nuclear localization were well conserved. In addition, the nucleotide and the functional amino acid sequences of a part of the HSV-2 conterpart of the HSV-1 ICP 18.5 gene were also compared, demonstrating an 88.4% and 95.9% homology, respectively.

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  1. Department of Virology, Faculty of Medicine, Kyushu University, Fukuoka, Japan

    Y. Toh, Y. Liu, S. Tanaka & R. Mori

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  1. Y. Toh
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  2. Y. Liu
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  4. R. Mori
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Toh, Y., Liu, Y., Tanaka, S. et al. Nucleotide sequence of the major DNA-binding protein gene of herpes simplex virus type 2 and a comparison with the type 1 counterpart. Archives of Virology 129, 183–196 (1993). https://doi.org/10.1007/BF01316894

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  • DOI: https://doi.org/10.1007/BF01316894

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