Elsevier

Virology

Volume 185, Issue 1, November 1991, Pages 500-504
Virology

Short communication
A comparison of the nucleotide sequences of cognate NS2 genes of three different orbiviruses

https://doi.org/10.1016/0042-6822(91)90808-OGet rights and content

Abstract

The genes encoding nonstructural protein NS2 of African horsesickness virus (AHSV) and epizootic hemorrhagic disease virus (EHDV) were cloned, sequenced, and compared to the NS2 gene of bluetongue virus (BTV). Nucleotide similarity ranged from 53 to 60%. The length of the proteins varied from 376 amino acids (EHDV) to 365 amino acids (AHSV). The N-terminal half of NS2 is more conserved (±58% similarity) among the three orbiviruses, while the C-terminal half contains a 120 amino acid region of low similarity (18%). The variable region has a high content of α-helix conformation and a hydrophilic character. A short region of 9 amino acids contains 5 amino acids that are either similar or identical in single-stranded RNA binding proteins of BTV, EHDV, AHSV, reovirus and rotavirus.

References (39)

  • H. Huismans

    Virology

    (1979)
  • H. Huismans et al.

    Virology

    (1979)
  • M.A. Devaney et al.

    Virus Res.

    (1988)
  • M.J. Grubman et al.

    Virology

    (1983)
  • H. Huismans et al.

    Virology

    (1976)
  • M.J. Grubman et al.

    Virus Res.

    (1990)
  • H. Huismans et al.

    Virology

    (1987)
  • D.G. Ritter et al.

    Virus Res.

    (1988)
  • L.W. Cashdollar et al.

    Virology

    (1984)
  • P.P.C. Mertens et al.

    Virology

    (1985)
  • Z. Xu et al.

    Virology

    (1989)
  • J.R. Wiener et al.

    Virology

    (1987)
  • J. Garnier et al.

    J. Mol. Biol.

    (1978)
  • G. Dreyfuss et al.

    Trends Biochem. Sci.

    (1988)
  • C.C. Query et al.

    Cell

    (1989)
  • I.W. Mattaj

    Cell

    (1989)
  • C.W. Ward et al.

    Virology

    (1984)
  • C.W. Bremer

    Onderstepoort J. Vet. Res.

    (1976)
  • H. Huismans et al.

    Onderstepoort J. Vet. Res.

    (1979)
  • Cited by (42)

    • African horse sickness virus serotype 4 antigens, VP1-1, VP2-2, VP4, VP7 and NS3, induce cytotoxic T cell responses in vitro

      2016, Virus Research
      Citation Excerpt :

      VP2 interacts with neutralizing antibodies during an infection and contains the majority of neutralising epitopes (Burrage et al., 1993; Roy, 1996). Non-structural proteins NS1, NS2, NS3, NS3a and NS4 have been identified in infected cells and are involved in replication, morphogenesis and release from the infected cells (van Staden et al., 1991; Belhouchet et al., 2011). The disease is controlled by preventative vaccination with a live, attenuated, multivalent commercial vaccines.

    • Vaccination of mice with a modified Vaccinia Ankara (MVA) virus expressing the African horse sickness virus (AHSV) capsid protein VP2 induces virus neutralising antibodies that confer protection against AHSV upon passive immunisation

      2014, Virus Research
      Citation Excerpt :

      The inner core encloses VP1, VP4 and VP6 proteins, (encoded by segments 1, 4 and 5, respectively) comprising the viral replicase complex and the 10 double-stranded RNA genome segments. There are four nonstructural proteins (NS1, NS2, NS3/3A, and NS4), involved in virus and morphogenesis (Belhouchet et al., 2011; Manole et al., 2012; Roy et al., 1994; van Staden et al., 1991). AHSV infection in horses most often results in severe clinical disease and death but those animals that survive exhibit a solid lifelong serotype-specific immunity.

    • Epizootic heamorragic disease

      2011, Research in Veterinary Science
      Citation Excerpt :

      Table 2 summarises the list of reference strains as widely accepted highlighting the urgent need of establishing reference strains for EHDV serotypes. Genomic studies and phylogenetic analyses also provided important information on the properties and origin of the strains (Wilson, 1994b; 1991a,b; van Staden et al., 1991; Cheney et al., 1996; Mecham et al., 2003; Murphy et al., 2005; 2006; Anthony et al., 2009a,b,c; 2010). The VP2 gene sequences of EHDV-3 (NIG1967/-1) and EHDV-1 shared an 88.7% identity (Anthony et al., 2009a) conversely other gene sequences showed low percentage of identity (Wilson et al., 2009b; Anthony et al., 2009b).

    View all citing articles on Scopus

    The nucleotide sequence data reported in this paper have been submitted to the GenBank nucleotide sequence database and have been assigned the accession numbers M 69090 (AHSV-NS2) and M 69091 (EHDV-NS2).

    1

    Present address: Animal & Dairy Science Research Institute, Irene Pretoria, South Africa.

    View full text