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High-resolution proteomic profiling of spider venom: expanding the toxin diversity of Phoneutria nigriventer venom

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Abstract

Here we present a proteomic characterization of Phoneutria nigriventer venom. A shotgun proteomic approach allowed the identification, for the first time, of O-glycosyl hydrolases (chitinases) in P. nigriventer venom. The electrophoretic profiles under nonreducing and reducing conditions, and protein identification by mass spectrometry, indicated the presence of oligomeric toxin structures in the venom. Complementary proteomic approaches allowed for a qualitative and semi-quantitative profiling of P. nigriventer venom complexity, expanding its known venom proteome diversity.

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Acknowledgments

This work was supported by grants from the Fundação de Amparo à Pesquisa do Estado de São Paulo (11/08514-8; 13/07467-1; 02/04545-7) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES).

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Correspondence to André Zelanis.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Handling Editor: M. S. Palma.

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Liberato, T., Troncone, L.R.P., Yamashiro, E.T. et al. High-resolution proteomic profiling of spider venom: expanding the toxin diversity of Phoneutria nigriventer venom. Amino Acids 48, 901–906 (2016). https://doi.org/10.1007/s00726-015-2151-6

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  • DOI: https://doi.org/10.1007/s00726-015-2151-6

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