Fungal Barcoding Primers

Fungal Barcoding Primer Sequences

This comprehensive table presents primer sequences integral to fungal barcoding studies, meticulously designed for accurate identification and classification of fungal species through DNA barcoding techniques. Covering key genomic regions such as the internal transcribed spacer (ITS), large subunit (LSU), small subunit (SSU), RNA polymerase II (RPB1 and RPB2), and minichromosome maintenance protein 7 (MCM7), these primers contribute to the 80% success rate observed in fungal species identification, facilitating advancements in fungal taxonomy and evolutionary studies.

Primer nameSequenceLab/commenterTax group

ITS
ITS1 5'-TCCGTAGGTGAACCTGCGG-3'forwardallall
ITS 1F5'-CTTGGTCATTTAGAGGAAGTAA-3'forwardallall
ITS45'-TCCTCCGCTTATTGATATGC-3'reverseallall
ITS4BR5'-TCAACAGACTTGTACATGGTCC-3'reverseHamelin
ITS4BR25'-GGATTATCACCCTCAATGAT-3'reverseHamelin
ITS3R25'-AAGGTACACCTGTTTGAGTGTC-3'forwardHamelin
ITS2R25'-GACACTCAAACAGGTGTACCTT-3'reverseHamelin
ITS55'-GGAAGTAAAAGTCGTAACAAGG-3'forwardSeifert
ITS3R35'-CACATCGATGAAGAACACAGT-3'forwardHamelin
5.8S5'-CGCTGCGTTCTTCATCG-3'forwardMoncalvo
5.8SR5'-TCGATGAAGAACGCAGCG-3'reverseMoncalvo
UN-UP18S425'-CGTAACAAGGTTTCCGTAGGTGAAC-3'forwardLevesque
UN-LO28S22`5'-GTTTCTTTTCCTCCGCTTATTGATATG-3'reverseLevesque

LSU
LR0R5'-ACCCGCTGAACTTAAGC-3'forward
LR55'-TCCTGAGGGAAACTTCG-3'reverse
LR165'-TTCCACCCAAACACTCG-3'reverse

SSU
SR1R5'-TACCTGGTTGATQCTGCCAGT-3'forwardJamesChytrids/Zygos, all
NS15'-GTAGTCATATGCTTGTCTC-3'forwardallAscomycota, all
NS45'-CTTCCGTCAATTCCTTTAAG-3'reverseallAscomycota, all
PNS15’-CCAAGCTTGAATTCGTAGTCATATGCTTGTC-3’forwardHibbettBasidiomycota
NS415’-CCCGTGTTGAGTCAAATTA-3’reverseHibbettBasidiomycota

RPB1
RPB1-Af, RPB1-Ac5’-GARTGYCCDGGDCAYTTYGG-3’forwardSung, Mathenyall
RPB1-Cr5’-CCNGCDATNTCRTTRTCCATRTA-3’reverseSung, Mathenyall

RPB2
fRPB2-5FGAYGAYMGWGATCAYTTYGGforwardMathenyall
RPB2-7RCCCATWGCYTGCTTMCCCATreverseSungall
bRPB2-7.1R5'-CCCATRGCYTGYTTMCCCATDGC-3'reverseMathenyBasidiomycota

MCM7
Mcm7-709for5’-ACIMGIGTITCVGAYGTHAARCC-3’forwardSchmittall
Mcm7-1348rev5’-GAYTTDGCIACICCIGGRTCWCCCAT-3’reverseSchmittall
Mcm7-1447rev5’-CATIACIGCIGCIGTRAGRCC-3’reverseSchmittall

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